![]() Non-covalently bound cell surface proteins, such as Slp and S-layer associated proteins (SLAPs) can be extracted from cells using denaturing salts such as LiCl. Notably, the S- layer forming strains (highlighted in pink box) phylogenetically cluster distinct from the non-S-layer forming lactobacilli (highlighted in blue box). The tree was made using the neighbor-joining method, rooted with L. ![]() ![]() Klaenhammer 1,2 1 Graduate Program in Microbiology, North Carolina State University, Raleigh, NC, USA 2 Department of Food, Bioprocessing, and Nutrition Science, North Carolina State University, Raleigh, NC, USA 250 kDa 150 kDa 100 kDa 75 kDa 50 kDa 37 kDa 25 kDa 20 kDa 15 kDa 10 kDa NCFM NCK 936 NCK 777 NCK 778 NCK 776 NCK 246 L. This opens new avenues for the selection of effective probiotics and the engineering of immunomodulatory bacteria. ![]() Using a modified LiCl extraction protocol coupled with LC-MSMS, we have proteomically identified the various extracellular proteins and SLAPs of the L.Īnalyses of these data highlight the proteomic complexity and differences of the cell surface of probiotic lactobacilli and reveal the potential for SLAPs to mediate intimate interactions with the intestinal mucosa. Based on previous data regarding the identification of S-layer associated proteins (SLAPs) in L.
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